ENOD40 Gene Expression and Cytokinin Responses in the Nonnodulating, Nonmycorrhizal (NodMyc) Mutant, Masym3, of Melilotus alba Desr.

Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (NodMyc) mutant Masym3.

We previously showed that applying 10(-6) M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod(-)Myc(-)Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots. In contrast, inoculation with a Nod(-)S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (...

Effect of Genotype and Growth Stage on Distribution of Melilotic Acid, o-Coumaric Acid, and Coumarinic Acid in Melilotus alba Desr

Investigation of Four Classes of Non-nodulating White Sweetclover (Melilotus alba annua Desr.) Mutants and Their Responses to Arbuscular-Mycorrhizal Fungi.

The nitrogen-fixing symbiosis between Rhizobiaceae and legumes is one of the best-studied interactions established between prokaryotes and eukaryotes. The plant develops root nodules in which the bacteria are housed, and atmospheric nitrogen is fixed into ammonia by the rhizobia and made available to the plant in exchange for carbon compounds. It has been hypothesized that this symbiosis evolve...

Comparison of Protein Constituents Relating to beta-Glucosidase Activity in BB and bb Inbred Lines of MELILOTUS ALBA.

I N biennial white-blossomed sweetclover (Melilotus alba Desr.) , the B/b allelic pair controls .&glucosidase activity ( SCHAEFFER, HASKINS and GORZ 1960; HASKINS and GORZ 1965). Homogenates of BB and Bb plants are high and iatermediate, respectively, in /3-glucosidase activity. No activity has been detected in homogenates of bb plants. The precise effect of the alleles B and b on the enzyme ha...

An open-flower mutant of Melilotus alba: Potential for floral-dip transformation of a papilionoid legume with a short life cycle?

This paper describes an open-flower mutant, designated opf, that we discovered in a genetic screen of fast neutron bombardment mutants in an attempt at floral-dip transformation of Melilotus alba (Fabaceae; white sweetclover), an alternative papilionoid legume host for Sinorhizobium meliloti. The opf mutant developed flowers with reflexed sepals and petals, thereby exposing the stamens and carp...